UPF3A monoclonal antibody (M06), clone 2E2
产品名称： UPF3A monoclonal antibody (M06), clone 2E2
英文名称： UPF3A monoclonal antibody (M06), clone 2E2
产品编号： H00065110-M06
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant UPF3A.
- Immunogen:
- UPF3A (AAH23569.1, 274 a.a. ~ 374 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- TGGGKQESCAPGAVVKARPMEGSLEEPQETSHSGSDKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPERLSRRSEDEQRWGKGPGQDRGKKGSQDSGAP
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of UPF3A expression in transfected 293T cell line by UPF3A monoclonal antibody (M06), clone 2E2.
Lane 1: UPF3A transfected lysate(51 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to UPF3A on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged UPF3A is 0.03 ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 65110
- GeneBank Accession#:
- BC023569
- Protein Accession#:
- AAH23569.1
- Gene Name:
- UPF3A
- Gene Alias:
- HUPF3A,RENT3A,UPF3
- Gene Description:
- UPF3 regulator of nonsense transcripts homolog A (yeast)
- Omim ID:
- 605530
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000018789,UPF3 regulator of nonsense transcripts homolog A